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KMID : 0364820120480040314
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Korean Journal of Microbiology
2012 Volume.48 No. 4 p.314 ~ p.318
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Virus Inactivation during the Manufacture of a Collagen Type I from Bovine Hides
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Bae Jung-Eun
Kim Chan-Kyong
Kim Sung-Po
Yang Eun-Kyung
Kim In-Seop
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Abstract
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Most types of collagen used for biomedical applications, such as cell therapy and tissue engineering, are derived
from animal tissues. Therefore, special precautions must be taken during the production of these proteins in order to
assure against the possibility of the products transmitting infectious diseases to the recipients. The ability to remove
and/or inactivate known and potential viral contaminants during the manufacturing process is an ever-increasingly
important parameter in assessing the safety of biomedical products. The purpose of this study was to evaluate the
efficacies of the 70% ethanol treatment and pepsin treatment at pH 2.0 for the inactivation of bovine viruses during
the manufacture of collagen type I from bovine hides. A variety of experimental model viruses for bovine viruses
including bovine herpes virus (BHV), bovine viral diarrhea virus (BVDV), bovine parainfluenza 3 virus (BPIV-3),
and bovine parvovirus (BPV), were chosen for the evaluation of viral inactivation efficacy. BHV, BVDV, BPIV-3,
and BPV were effectively inactivated to undetectable levels within 1 h of 70% ethanol treatment for 24 h, with log
reduction factors of ≥5.58, ≥5.32, ≥5.11, and ≥3.42, respectively. BHV, BVDV, BPIV-3, and BPV were also
effectively inactivated to undetectable levels within 5 days of pepsin treatment for 14 days, with the log reduction
factors of ≥7.08, ≥6.60, ≥5.60, and ≥3.59, respectively. The cumulative virus reduction factors of BHV, BVDV,
BPIV-3, and BPV were ≥12.66, ≥11.92, ≥10.71, and ≥7.01. These results indicate that the production process for
collagen type I from bovine hides has a sufficient virus-reducing capacity to achieve a high margin of virus safety
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KEYWORD
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bovine collagen, ethanol treatment, pepsin treatment, virus safety
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